Effect of extender on the quality and incubation resilience of cryopreserved Holstein bull semen

There are still concerns over sperm quality when plant-based diluents used instead of animal-based semen extenders. Therefore, in our study we compared the effects one soybean lecithin-based (AndroMed®) and two egg yolk-based (BullXcell® Optidyl®) commercially available extenders on post-thaw vitro quality. Fifty ejaculates collected from ten bulls were aliquoted into three parts diluted with above-mentioned Post-thaw viability, mitochondrial membrane potential (MMP), plasma integrity, acrosome status analysed immediately after thawing (0 h) at an hourly interval during 2-h incubation 38 °C. Sperm functionality variables assessed by simultaneous quadruple staining using flow cytometry. Semen stored Optidyl® had a greater integrity than that AndroMed® BullXcell® (P < 0.05). With use there was higher percentage high MMP 0.05) other The affected development parameters differently as related to showed increase, while decline. Although all responded treatments similar manner, significant intra- inter-male differences found. In conclusion, results clearly displayed beneficial yolk lecithin supplementation